Frequently Asked Questions
Who do I contact to set up a project with the CGL?
Please contact cgl@mdanderson.org.
What kinds of specimens/samples can I submit and deliver to CGL?
You can find information on the types of specimens and samples that are accepted by the CGL on our main landing page.
How do I extract DNA and/or RNA?
For projects with more than 50 samples, DNA and RNA extractions must be done through the Biospecimen Extraction Facility. Projects with 50 samples or fewer may have DNA and RNA extracted within CGL. Specimen submission for extraction must specify on the CGL submission form whether to extract the specimen(s) for DNA or RNA.
Sample Submission Questions
I don¡¯t have access to CGL Data Portal. How do I get access?
Send an email to MMMohammad@mdanderson.org with the following information to request user account:
- Primary Investigator¡¯s first and last name
- Primary Investigator¡¯s MD Anderson username
How do I submit samples to CGL?
Samples may be submitted to the CGL by contacting cgl@mdanderson.org. Please contact in advance of sample submission to ensure availability to receive and review your samples. Please use the appropriate sample submission form. You may hand deliver your samples, or you can use the MD Anderson courier to deliver your samples to CGL.
Sample Submission Forms
Please choose the appropriate form to download and fill out:
- Download the form for MD Anderson samples
- Download the form for non-MD Anderson samples (for external projects)
What total amount, volume, quality, and buffer do I need for submitting DNA and/or RNA?
Please see our tables for minimum (determined by PicoGreen) amount, volume, and quality for processing. We typically request that stock samples of DNA and RNA be submitted, and we will return all remaining excess sample to the investigator. DNA and RNA quantities and concentrations determined by Nanodrop will typically be 2X-5X higher (and inaccurate) compared to the more accurate quantities and concentrations determined by PicoGreen. The CGL accepts both DNA and RNA samples in low TE buffer (1x Low TE: 10 mM Tris-HCl (pH8.0) + 0.1 mM EDTA).
How long is the turn-around time for sample QC?
Sample turnaround time is dependent upon queue length, as samples are run in the order they are received. If there are standard queues when samples are submitted, and there are ¡Ü50 samples submitted, turnaround time per platform is provided below.
Projected timelines for completion of NextGen sequencing are affected by multiple factors. There are queues for each part of the sequencing pipeline, from nucleic acid extraction and quality control (QC), library prep and capture to bioinformatics¡¯ analysis. These queues are determined by the volume of samples that were submitted but have not yet been processed for any particular step of the pipeline.
Sequencing turnaround time starts after receiving the PI¡¯s approval of the DNA/RNA QC results. We recommend that all investigators adequately plan for foreseeable deadlines and complicating factors that may include upcoming conferences, publication deadlines, grant submission deadlines and grant funding deadlines.
Platform |
Turnaround time from platform approval to data receipt |
---|---|
DNA/RNA Extraction |
Depends on the pipeline business, but usually 10 business days |
DNA/RNA QC |
Depends on the pipeline business, but usually 10 business days |
Routine NGS-platforms |
12 to 16 weeks |
What is the shipping address for the CGL for sample submission?
Cancer Genomics Laboratory (CGL)
c/o Marcus Coyle
Zayed Z4.3010
MD Anderson Cancer Center
6565 M.D. Anderson Blvd.
Houston, TX 77030
Can I develop a custom experimental design?
We can develop custom experimental designs for the NGS platform. Please contact mmmohammad@mdanderson.org for more details.