Instrumentation and Software
Instruments
Serial dilution and transfer of lysates to master plates
Printing of samples from master plates to nitrocellulose slides
Staining slides for detection of spot (sample) intensities
Scanning slides for digital reproduction of spot (sample) intensities
Scanning slides for digital conversion of stained slides
Software
Used for spot identification and quantification using Mean Net and Regional Background Correction.
Used for curve fitting of samples and determination of protein levels
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