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Extracellular vesicles (EVs) from cerebrospinal fluid (CSF), saliva, tissue, milk, blood, lymph, bile, urine or cell culture supernatant are isolated and purified using SE-FPLC and other technologies. The EVs can be analyzed in several ways, including nanoparticle tracking analysis, single EV analysis and sorting, Western blotting and microscopy. EV engineering for preclinical studies is also provided. First cells are grown in bioreactors, then pooled, centrifuged and re-suspended. EVs are extracted, and payload can be loaded. They are then prepared for administration in?preclinical settings.